Sat, Mar 12 – Dynamic DNA

Full Day [8 am – 4 pm ] 0.5 sem hrs / 7.5 contact hours – In Person

3.12 March PALS Flyer

Presenters: Terrie Watts, Science Teacher, Discovery Canyon Campus, Megan Caraway, Science
Teacher, Vista Ridge High School, Jane Wilson, Science Teacher, Falcon Homeschool
Program

Location: Discovery Canyon Campus
Description: All organisms have it. It contains all of the information necessary to build and maintain
an organism. It can help solve crimes. It can link us to our ancestors. What is it? DNA –
of course! In this workshop, you will engage in activities focusing on DNA. You will
perform PCR and gel electrophoresis to learn the importance of these techniques to our
understanding of this amazingly dynamic molecule. You will also participate in a variety
of engaging genetic- based activities that you can easily implement in your life science
classes.

Agenda: 8:00 Welcome, Introductions, Administrivia

8:15 – 9:15 DNA Analysis – Intro & PCR

● Introduction – Handout – from a Kit – PCR –
● Practice Micropipetting [30 min]
● PCR set up [30 min], run [2 – 2.5 hours]

9:30 – 11:00 DNA Activities

● Explore – DNA extractions
● Paper Electrophoresis (CAT simulation?? – paper exercise – models
electrophoresis is doing?)

11:00 – 12:00 DNA Analysis – Gel Electrophoresis Prep

● Practice micropipetting with dummy gel [30 min] – dry and submerged in
water & load get
● Electrophoresis run (30-45 minutes) with Fluorescent dye in gel

12:00 – 12:30 Lunch – network
12:30 – 1:00 DNA Analysis – Gel Electrophoresis

● View under uv boxes. & do analysis [30 min]

1:00 – 2:30 Genetic Activities
● Create a Creature
● Putting it all together- Pedigrees

2:30 – 3:30 Genetic Engineering and current DNA research
3:30 -4 Evaluations and doorprizes